Acinetobacter calcoaceticus-Acinetobacter baumannii complex in animals: identification and antimicrobial resistance profile

ABSTRACT: Acinetobacter spp. is emerging as an important human and veterinary pathogen, mostly due to intrinsic acquired resistance antimicrobials. Despite its public health relevance, little known about the prevalence, role of different species antimicrobial profile animal-origin isolates. Traditional phenotypic tests may fail discriminate species, therefore molecular analyses are often required a complementary approach. The objectives this study were evaluate occurrence strains calcoaceticus-Acinetobacter baumannii (Acb) complex isolated from animal infections including urinary tract infections, otitis, piodermitis pododermatitis, against classes, carbapenems. All Gram-negative coccobacilli isolates characterized by MALDI-TOF multiplex PCR, disk diffusion test was used investigate multi-drug (MDR) carbapenem genes PCR preconized standard guidelines. technique identified 21 belonging Acb (10 A. pittii, 8 baumannii, 3 nosocomialis, 1 ursingii, venetianus). Multiplex confirmed results for 20 strains. Eight (34.78%) classified MDR, being 50% (4/8) 37.5% (3/8) 12.5% (1/8) nosocomialis. None presented carbapenemase production. Considering genes, 26.09% (6/23) one or more genes. From these, (3/6) only bla VIM, 33.33% (2/6) blaIMP, 16.67% (1/6) blaIMP e blaVIM, simultaneously. These detected among pittii (66.67%, 4/6). This provides further insights into origin.